Generation of human induced pluripotent cells on a human feeder layer
نویسندگان
چکیده
منابع مشابه
efficient differentiation of human induced pluripotent stem cell (hipsc) derived hepatocyte-like cells on hmscs feeder.
background: the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. differentiation of human induced pluripotent stem cells (hipscs) to hepatocyte-like cells (hlcs) on mouse embryonic fibroblasts (mefs) feeders is confronted with several problems that hinder the clinical applications of these differentiated cells for the treatment of liver injuries. ...
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background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...
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The optimal maintenance of human embryonic stem cells (hESC) in vitro is generally observed in the presence of a feeder-layer of mouse embryonic fibroblasts in a serum-containing medium. Various approaches are now available to remove the feeder requirement. Today, the best feeder-free system for the maintenance of hESC and induced pluripotent stem (iPS) cells is based on a serum-replacement med...
متن کاملSpecification of Hemato-Endothelial-Like Structures and Generation of Hematopoietic Progenitor Cells from Human Pluripotent Stem Cells
Background and purpose: Human pluripotent stem cells (hPSCs) with the ability to differentiate into adult cells have provided a new perspective for treatment of some diseases. But, the efficiency of differentiation methods to generate hematopoietic progenitor cells (HPCs) is faced with multiple challenges. In the present study, we investigated the formation of hemato-endothelial-like structure...
متن کاملEfficient Differentiation of Human Induced Pluripotent Stem Cell (hiPSC) Derived Hepatocyte-Like Cells on hMSCs Feeder
BACKGROUND The use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. Differentiation of human induced Pluripotent Stem Cells (hiPSCs) to Hepatocyte-like Cells (HLCs) on mouse embryonic fibroblasts (MEFs) feeders is confronted with several problems that hinder the clinical applications of these differentiated cells for the treatment of liver injuries. S...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61212-6